New monoclonal antibodies against the putative immunosuppressive site of retroviral p15E
article
Both retroviral infections as well as human tumors may cause immunosuppression. One of the factors involved in immunosuppression in patients with squamous cell carcinoma of the head and neck (SCC-HN) is a protein related in the retroviral protein p15E. A conserved, 17-amino acid sequence represents the immunosuppressive epitope of retroviral p15E. In order to study the relationship between SCC-HN associated immunosuppression and retroviral p15E, we produced three new monoclonal antibodies (MAbs: ER- IS1, ER-IS2, and ER-185) directed against the immunosuppressive synthetic CKS-17 peptide. These MAbs react with the immunosuppressive peptide (in enzyme-linked immunosorbent assay), with human tumor cell lines (in FACScan analysis), with retroviral p15E (on Western blot), and with cryostat sections of SCC-HN tumor tissue. In addition, the MAbs neutralize the immunosuppressive low molecular weight factors present in sera of patients with SCC-HN. These results show that retroviral p15E and the immunosuppressive factors associated with SCC-HN share a conserved immunosuppressive epitope and that MAbs against this epitope can be used for detection and centralization of the tumor-associated immunosuppressive protein(s).
Topics
Monoclonal antibodyMonoclonal antibody er is1Monoclonal antibody er is2Monoclonal antibody er is5Unclassified drugAirus proteinAntibody productionAntigen antibody reactionAntigen recognitionCancer cell cultureCancer immunotherapyHead and neck cancerHumanHuman cellRetrovirusSquamous cell carcinomaVirus infectionAmino Acid SequenceAntibodies, MonoclonalBinding Sites, AntibodyBlotting, WesternCarcinoma, Squamous CellCell LineConserved SequenceEnzyme-Linked Immunosorbent AssayEpitopesHead and Neck NeoplasmsImmunoblottingImmunohistochemistryImmunosuppressionLaryngeal NeoplasmsLung NeoplasmsLymphoma, Large-CellMolecular Sequence DataPeptidesRauscher VirusRetroviridaeRetroviridae Proteins, OncogenicTumor Cells, CulturedAntibodies, MonoclonalBinding Sites, AntibodyEpitopes
TNO Identifier
280590
ISSN
0008-5472
Source
Cancer Research, 54(7), pp. 1831-1836.
Pages
1831-1836
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