Evaluation of an alternative in vitro test battery for detecting reproductive toxicants in a grouping context
article
Previously we showed a battery consisting of CALUX transcriptional activation assays, the ReProGlo assay, and the embryonic stem cell test, and zebrafish embryotoxicity assay as 'apical' tests to correctly predict developmental toxicity for 11 out of 12 compounds, and to explain the one false negative [7]. Here we report on applying this battery within the context of grouping and read across, put forward as a potential tool to fill data gaps and avoid animal testing, to distinguish in vivo non- or weak developmental toxicants from potent developmental toxicants within groups of structural analogs. The battery correctly distinguished 2-methylhexanoic acid, monomethyl phthalate, and monobutyltin trichloride as non- or weak developmental toxicants from structurally related developmental toxicants valproic acid, mono-ethylhexyl phthalate, and tributyltin chloride, respectively, and, therefore, holds promise as a biological verification model in grouping and read across approaches. The relevance of toxicokinetic information is indicated. © 2014 Elsevier Inc.
Topics
Alternative modelCALUXDevelopmental toxicityEmbryonic stem cell testGroupingRead acrossReProGloZebrafish embryotoxicity assay2 ethylhexanoic acid2 methylhexanoic acidDibutyltin dichloridemonobutyltin trichlorideMonoethylhexyl phthalateMonomethyl phthalateToxic substanceTributyltin chlorideUnclassified drugValproic acidAnalytic methodAnimal cellAssayCALUX batteryChemical analysisChemical proceduresChemical structureControlled studyDevelopmental toxicityDosimetryEmbryonic stem cell testEmbryotoxicityFertilizationIn vitro studyMorphologyMouseNonhumanReProGlo assayToxicokineticsZebrafish embryotoxicity testAnimaliaDanio rerio
TNO Identifier
527795
ISSN
08906238
Source
Reproductive Toxicology, 55, pp. 11-19.
Pages
11-19
Files
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