To pool or not to pool? Impact of the use of individual and pooled fecal samples for in vitro fermentation studies

article



Aguirre, M.

Ramiro-Garcia, J.

Koenen, M.E.

Venema, K.
This study investigated the stability and the activity of the microbiota from a single and a pool of donors in the TNO in vitro model of the colon (TIM-2 system). Our findings demonstrate the suitability of the preparation of a pool of fecal sample to be used for fermentation experiments.
Chemicals/CAS: isobutyric acid, 5711-69-3, 79-31-2; isovaleric acid, 503-74-2, 539-66-2
Topics

































Fecal inoculum preparationFermentationGut microbiotaIn vitro modelPoolingBranched chain fatty acidFatty acidIsobutyric acidIsovaleric acidShort chain fatty acidUnclassified drugActinobacteriaAdultBacteroidetesClostridiumControlled studyFecesFemaleFermentation techniqueFirmicutesGenusHumanHuman experimentIn vitro studyInoculationIntestine floraMicrobial communityNonhumanNormal humanPrevotellaProteobacteriaSampleTuricibacterVerrucomicrobia
TNO Identifier
517662
Repository link
https://resolver.tno.nl/uuid:2570268d-3fe9-44b7-99a3-26550848c348
ISSN
01677012
Source
Journal of Microbiological Methods, 107, pp. 1-7.
Pages
1-7
Files
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