Phase-system switching: a new approach in liquid chromatography/mass spectrometry coupling
article
A new approach is presented to overcome the problems imposed by the use of non‐volatile mobile phase constituents, such as buffers, in liquid chromatographic/mass spectrometric applications. The approach, called phase‐system switching (PSS), is based on the use of valve switching techniques. The peak of interest is heart‐cut from the effluent of the analytical column and trapped on a short trapping column, the buffer constituents are washed off, and subsequently the compound of interest is desorbed in a solvent favourable for the liquid chromatograph/mass spectrometer interface. The potential of the phase‐system switching technique is explored in the liquid chromatographic/mass spectrometric analysis of the anti‐cancer drug mitomycin C, which is chromatographed with an aqueous phosphate buffer as the mobile phase on a reversed‐phase column. In addition to the phase‐system switching itself, the PSS technique shows the interesting possibilities of peak compression and changes in flow rate.
TNO Identifier
247069
Source
Biomedical and environmental mass spectrometry, 16(1-2), pp. 393-397.
Pages
393-397
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