The sequence A-alpha-(148-160) in fibrin, but not in fibrinogen, is accessible to monoclonal antibodies
article
Fibrin, but not fibrinogen, accelerates the activation of plasminogen catalyzed by tissue-type plasminogen activator. Previous work showed that essential information for this accelerating capacity of fibrin resides in the sequence corresponding to residues 148-160 of the Aa chain of fibrinogen [Aa-(148-160)]. Our working hypothesis, based on those findings, is that Aa-(148-160) is buried in fibrinogen and becomes accessible to proteins such as plasminogen and/or tissue-type plasminogen activator when fibrinogen is converted to fibrin. To test this hypothesis we have raised a monoclonal antibody against synthetic Aa-(148-160) and found that this antibody reacts with fibrin and not with fibrinogen. This finding shows that Aa-(148-160) becomes accessible when fibrinogen is converted to fibrin and that Aa-(148-160) is a fibrin-specific neoantigenic determinant.
TNO Identifier
244063
Source
Proceedings National Academy of sciences USA, 86, pp. 8951-8954.
Pages
8951-8954
Files
To receive the publication files, please send an e-mail request to TNO Repository.