A method for the transformation of hybridoma cell lines with improved efficiency : its use in the production of bispecific monoclonal antibodies
bookPart
A method was investigated to transduce a specific drug resistance marker, coded on a bacterial plasmid construct, to a hybridoma cell line using electroporation. We tested the influence of several buffers and the form of the DNA on cell viability and stable transfection frequency. We found a tenfold higher stable transfection frequency using DMEM + FCS as eiectroporation buffer, as compared with HBS. Furthermore, using DMEM + FCS, plasmid linearisation had little effect on the stable transfection frequency. This method proved to be succesful when isolating quadromas for the production of bispecific antibodies.
TNO Identifier
243514
Publisher
Kluwer
Source title
From clone to clinic
Editor(s)
Crommelin, D.J.A.
Schellekens, H.
Schellekens, H.
Pages
201-207
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