Preparation and Evaluation of Lignocellulosic Biomass Hydrolysates for Growth by Ethanologenic Yeasts
bookPart
Lignocellulosic biomass is a potential feedstock for bioethanol production. Biomass hydrolysates, prepared with a procedure including pretreatment and hydrolysis, are considered to be used as fermentation media for microorganisms, such as yeast. During the hydrolysate preparation procedure, toxic compounds are released or formed which may inhibit the growth of the microorganism and thus the product formation. To study the effects of these compounds on fermentation performance, the production of various hydrolysates with diverse inhibitory effects is of importance. A platform of methods that generates hydrolysates through four different ways and tests their inhibitory effects using Bioscreen C Analyzer growth tests is described here. The four methods, based on concentrated acid, dilute acid, mild alkaline and alkaline/ oxidative conditions, were used to prepare hydrolysates from six different biomass sources. The resulting
24 hydrolysates showed great diversity on growth rate in Bioscreen C Analyzer growth tests. The approach allows the prediction of a specifi c hydrolysate’s performance and helps to select biomass type and hydrolysate preparation method for a specifi c production strain, or vice versa.
24 hydrolysates showed great diversity on growth rate in Bioscreen C Analyzer growth tests. The approach allows the prediction of a specifi c hydrolysate’s performance and helps to select biomass type and hydrolysate preparation method for a specifi c production strain, or vice versa.
TNO Identifier
465570
Publisher
Springer Science and Business Media
Article nr.
Chapter 16
Source title
Microbial Metabolic Engineering: Methods and Protocols, DOI 10.1007/978-1-61779-483-4_16, © Springer Science+Business Media, LLC 2012
Editor(s)
Cheng, Q.
Pages
245-259
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