Characterization of antibacterial polyethersulfone membranes using the respiration activity monitoring system (RAMOS)
article
Membranes with antibacterial properties were developed using surface modification of
polyethersulfone ultrafiltration membranes. Three different modification strategies using
polyelectrolyte layer-by-layer (LbL) technique are described. The first strategy relying on
the intrinsic antibacterial properties of poly(diallyldimethylammonium chloride) (PDADMAC)
and poly(ethylenimine) (PEI) exhibits only little antibacterial effects. The other two
strategies contain silver in both ionic (Agþ) and metallic (Ag0) form. Agþ embedded into
negatively charged poly(sodium 4-styrene sulfonate) (PSS) layers totally inhibits bacterial
growth. Ag0 nanoparticles were introduced to the membrane surface by LbL deposition of
chitosan- and poly(methacrylic acid) e sodium salt (PMA)-capped silver nanoparticles and
subsequent UV or heat treatment. Antibacterial properties of the modified membranes
were quantified by a new method based on the Respiration Activity Monitoring System
(RAMOS), whereby the oxygen transfer rates (OTR) of E. coli K12 cultures on the membranes
were monitored online. As opposed to colony forming counting method RAMOS yields
more quantitative and reliable data on the antibacterial effect of membrane modification.
Ag-imprinted polyelectrolyte film composed of chitosan (Ag0)/PMA(Ag0)/chitosan(Ag0) was
found to be the most promising among the tested membranes. Further investigation
revealed that the concentration and equal distribution of silver in the membrane surface
plays an important role in bacterial growth inhibition
polyethersulfone ultrafiltration membranes. Three different modification strategies using
polyelectrolyte layer-by-layer (LbL) technique are described. The first strategy relying on
the intrinsic antibacterial properties of poly(diallyldimethylammonium chloride) (PDADMAC)
and poly(ethylenimine) (PEI) exhibits only little antibacterial effects. The other two
strategies contain silver in both ionic (Agþ) and metallic (Ag0) form. Agþ embedded into
negatively charged poly(sodium 4-styrene sulfonate) (PSS) layers totally inhibits bacterial
growth. Ag0 nanoparticles were introduced to the membrane surface by LbL deposition of
chitosan- and poly(methacrylic acid) e sodium salt (PMA)-capped silver nanoparticles and
subsequent UV or heat treatment. Antibacterial properties of the modified membranes
were quantified by a new method based on the Respiration Activity Monitoring System
(RAMOS), whereby the oxygen transfer rates (OTR) of E. coli K12 cultures on the membranes
were monitored online. As opposed to colony forming counting method RAMOS yields
more quantitative and reliable data on the antibacterial effect of membrane modification.
Ag-imprinted polyelectrolyte film composed of chitosan (Ag0)/PMA(Ag0)/chitosan(Ag0) was
found to be the most promising among the tested membranes. Further investigation
revealed that the concentration and equal distribution of silver in the membrane surface
plays an important role in bacterial growth inhibition
Topics
TNO Identifier
464255
ISSN
00431354
Source
Water Research, 46(16), pp. 5401-5409.
Pages
5401-5409
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