The role of cyclic nucleotides in the release of tissue-type plasminogen activator and von Willebrand factor
article
The modulation of the induced acute release of tissue-type plasminogen activator (t-PA) and of von Willebrand factor (vWF) by compounds affecting cyclic nucleotide levels was studied, using an isolated rat hindleg perfusion system. Platelet-activating factor (PAF; 5 nM) or bradykinin (0.8 μM) were used to induce release of t-PA and vWF. The guanylate cyclase activators sodium nitroprusside and atrial natriuretic factor reduced the induced release of t-PA and vWF. Release was not affected by inhibiting nitric oxide production with N(G)-nitro-L-arginine. The effects of nitroprusside and atrial natriuretic factor could not be reproduced by infusion of 8-bromo-cGMP. The adenylate cyclase activator forskolin had no effect on bradykinin-induced release of t-PA and vWF, reduced PAF-induced t-PA release, but potentiated PAF-induced vWF release. These modulatory effects were only partially mimicked by infusion of 8-bromo-cAMP. None of the compounds tested was able to induce the release of t-PA or of vWF in the absence of stimulation by bradykinin or platelet-activating factor. Cyclic nucleotides can thus modulate, but not induce, the acute release of t-PA and vWF from perfused rat hindlegs. Chemicals/CAS: 8 bromo cyclic AMP, 23583-48-4; adenylate cyclase, 9012-42-4; atrial natriuretic factor, 85637-73-6; bradykinin, 58-82-2, 5979-11-3; forskolin, 66575-29-9; guanylate cyclase, 9054-75-5; n(g) nitroarginine, 2149-70-4; nitric oxide, 10102-43-9; nitroprusside sodium, 14402-89-2, 15078-28-1; thrombocyte activating factor, 64176-80-3, 65154-06-5; tissue plasminogen activator, 105913-11-9; von Willebrand factor, 109319-16-6; 8-Bromo Cyclic Adenosine Monophosphate, 23583-48-4; 8-bromocyclic GMP, 31356-94-2; Adenylate Cyclase, EC 4.6.1.1; Atrial Natriuretic Factor, 85637-73-6; Bradykinin, 58-82-2; Cyclic AMP, 60-92-4; Cyclic GMP, 7665-99-8; Forskolin, 66428-89-5; Guanylate Cyclase, EC 4.6.1.2; Nitroprusside, 15078-28-1; Platelet Activating Factor; Tissue Plasminogen Activator, EC 3.4.21.68; von Willebrand Factor
Topics
8 bromo cyclic ampadenylate cyclaseatrial natriuretic factorbradykinincyclic nucleotideforskolinguanylate cyclasen(g) nitroargininenitric oxidenitroprusside sodiumthrombocyte activating factoranimal experimentcontrolled studyenzyme activationenzyme inhibitionhindlimbnonhumanperfusionpriority journalprotein secretion8-Bromo Cyclic Adenosine MonophosphateAdenylate CyclaseAnimalAtrial Natriuretic FactorBradykininCyclic AMPCyclic GMPEndothelium, VascularForskolinGuanylate CyclaseMaleNitroprussidePlatelet Activating FactorRatsRats, WistarSecretory RateTissue Plasminogen Activatorvon Willebrand Factor
TNO Identifier
232136
Source
Thrombosis and Haemostasis, 69(3), pp. 259-261.
Pages
259-261
Files
To receive the publication files, please send an e-mail request to TNO Repository.