Isolation of bifidobacteria for blood group secretor status targeted personalised nutrition
article
Background: Currently, there is a constant need to find microbial products for maintaining or even improving host microbiota balance that could be targeted to a selected consumer group. Blood group secretor status, determining the ABO status, could be used to stratify the consumer group.
Objective: We have applied a validated upper intestinal tract model (TIM-1) and culturing methods to screen potential probiotic bacteria from faeces of blood secretor and non-secretor individuals.
Design: Faecal samples from healthy volunteers were pooled to age- and sex-matched secretor and nonsecretor pools. Faecal pools were run through separate TIM-1 simulations, and bacteria were cultivated from samples taken at different stages of simulations for characterisation.
Results: Microbes in secretor pool survived the transit through TIM-1 system better than microbes of nonsecretor pool, especially bifidobacteria and anaerobes were highly affected. The differences in numbers of bifidobacteria and lactobacilli isolates after plate cultivations and further the number of distinct RAPDgenotypes was clearly lower in non-secretor pool than in secretor pool.
Conclusions: In the present study, we showed that microbiota of secretor and non-secretor individuals tolerate gastrointestinal conditions differently and that a combination of gastrointestinal simulations and cultivation methods proved to be a promising tool for isolating potentially probiotic bacteria.
Objective: We have applied a validated upper intestinal tract model (TIM-1) and culturing methods to screen potential probiotic bacteria from faeces of blood secretor and non-secretor individuals.
Design: Faecal samples from healthy volunteers were pooled to age- and sex-matched secretor and nonsecretor pools. Faecal pools were run through separate TIM-1 simulations, and bacteria were cultivated from samples taken at different stages of simulations for characterisation.
Results: Microbes in secretor pool survived the transit through TIM-1 system better than microbes of nonsecretor pool, especially bifidobacteria and anaerobes were highly affected. The differences in numbers of bifidobacteria and lactobacilli isolates after plate cultivations and further the number of distinct RAPDgenotypes was clearly lower in non-secretor pool than in secretor pool.
Conclusions: In the present study, we showed that microbiota of secretor and non-secretor individuals tolerate gastrointestinal conditions differently and that a combination of gastrointestinal simulations and cultivation methods proved to be a promising tool for isolating potentially probiotic bacteria.
TNO Identifier
462621
Source
Microbial Ecology in Health & Disease MEHD, 23, pp. 28-34.
Pages
28-34