Constitutive expression of LY-6.A2 on murine keratinocytes and inducible expression on TCRγδ+ dendritic epidermal T cells
article
We investigated the expression of Ly-6.A2 on isolated murine epidermal cells by flow cytometry. Ly-6.A2 was expressed on 61% of keratinocytes and 6% of dendritic epidermal T cells of C57BL mice. Phosphatidylinositol-specific phospholipase C removed Ly-6.A2, indicating that the antigen is anchored to the keratinocyte membrane via a glycosyl-phosphatidylinositol anchor similar to its attachment to the membrane of lymphocytes. Induction of dermatitis by topical application of PMA increased the expression of Ly-6.A2 on TCRγδ+ dendritic epidermal T cells and did not change its expression on keratinocytes. The increased expression of Ly-6.A2 on dendritic epidermal T cells was transient and reached a peak at 4 days after application of PMA, when 55% of the cells were positive. Chemicals/CAS: phorbol 13 acetate 12 myristate, 16561-29-8; phospholipase C, 9001-86-9; 1-phosphatidylinositol phosphodiesterase, EC 3.1.4.10; Antibodies, Monoclonal; Antigens, Ly; Antigens, Surface; Antigens, Thy-1; Membrane Glycoproteins; Phosphoric Diester Hydrolases, EC 3.1.4; Receptors, Antigen, T-Cell, gamma-delta; Tetradecanoylphorbol Acetate, 16561-29-8.
Topics
phorbol 13 acetate 12 myristatephorbol esterphospholipase canimal tissuearticledendritic cellflow cytometrygene expressionkeratinocytemalemousenonhumant lymphocyteAnimalAntibodies, MonoclonalAntigens, LyAntigens, SurfaceAntigens, Thy-1Cell MembraneDendritic CellsEpidermisFlow CytometryGene Expression RegulationImmunohistochemistryKeratinocytesMaleMembrane GlycoproteinsMiceMice, Inbred BALB CMice, Inbred C3HMice, Inbred C57BLMice, Inbred DBAPhosphoric Diester HydrolasesReceptors, Antigen, T-Cell, gamma-deltaT-LymphocytesTetradecanoylphorbol AcetateUp-Regulation
TNO Identifier
232090
ISSN
09231811
Source
Journal of Dermatological Science, 5(2), pp. 114-121.
Pages
114-121
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