The acquisition of a memory phenotype by murine CD4+ T cells is accompanied by a loss in their capacity to increase intracellular calcium.
article
During the process of aging, the fraction of CD4+ T cells with a naive phenotype, that is, Pgp-1- CD45RBHighMEL-14+, decreases in favor of CD4+ T memory cells. Total CD4+ T cells from aged mice displayed a diminished calcium response to anti-CD3 and even ionomycin as compared to the cells from young mice, and this was related to the changed composition of the CD4+ T-cell population. Regardless the age of the donor mice, naive CD4+ T cells effectively increased intracellular calcium, whereas memory CD4+ T cells were impaired in this regard. In addition, a heterogeneity in the differentiation stage of the naive CD4+ T cells was shown by the observation that calcium mobilization in naive CD4+ T cells from young mice was more profound than that in their aged counterparts. These data thus indicate that during the acquisition of a memory phenotype, murine CD4+ T cells lose the capacity to increase intracellular calcium, which in turn may be responsible for the decreased level of IL-2 production by these cells. Chemicals/CAS: calcium, 7440-70-2; ionomycin, 56092-81-0; Antibodies, Monoclonal; Antigens, CD3; Antigens, CD45; Calcium, 7440-70-2; Ionomycin, 56092-81-0
Topics
calciumCD3 antigenCD45 antigenionomycinmonoclonal antibodyaginganimalarticledrug effectimmunological memoryimmunologyimmunophenotypingmalemetabolismmousemouse strainphysiologyT lymphocyteAgingAnimalAntibodies, MonoclonalAntigens, CD3Antigens, CD45CalciumCD4-Positive T-LymphocytesImmunologic MemoryImmunophenotypingIonomycinMaleMiceMice, Inbred CBA
TNO Identifier
232032
ISSN
10446672
Source
Developmental immunology, 3(1), pp. 25-34.
Pages
25-34
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