Anther and microspore culture of Hordeum vulgare L. cv. Igri
article
The influence of environmental and cultural changes was investigated on both anther culture and microspore culture of barley cv. Igri. The highest regeneration frequency for both culture systems was obtained when at least 50% of the microspore population was in the mid-late to late uninucleate stage, when the anthers had been pretreated for 4 days on mannitol and when culture was performed with oxygen supply at regular intervals. Furthermore, the supplement of vitamins and casein hydrolysate in the culture medium improved the isolated microspore culture, whereas these compounds had a negative effect, if any effect at all, on the culture of anthers. Anther culture is not as laborious as microspore culture, but turned out to be at least 5 times less efficient. When mechanically isolated microspores were cultured, under the conditions found to be optimal in the present study, a mean of 12.4 green plants per anther was obtained. © 1992.
TNO Identifier
232030
ISSN
01689452
Source
Plant Science, 86(1), pp. 89-96.
Pages
89-96
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