The promoter of the rice gene GOS2 is active in various different monocot tissues and binds rice nuclear factor ASF-1
article
A single copy gene has been isolated, termed GOS2, from rice. Sequence comparison revealed highly similar genes in mammals and yeast, indicating that GOS2 encodes an evolutionary conserved protein. GOS2 mRNA was detected in all tissues examined. When the upstream region was translationally fused to the reporter gene gusA it was found to drive expression in a variety of rice tissues and in cell suspensions of other monocot species following introduction by particle bombardment. Therefore, the GOS2 promoter is potentially useful for genetic engineering of monocots. A DNA-binding activity from rice, termed rice ASF-1, with similar binding specificity as the cloned tobacco transcription factor TGA-1a, was found to bind to a TGACG sequence motif in the GOS2 promoter. Possible roles for rice ASF-1 in the transcriptional activation of the GOS2 promoter are discussed. Chemicals/CAS: beta glucuronidase, 9001-45-0; Glucuronidase, EC 3.2.1.31; GOS2 protein, Oryza sativa; Nuclear Proteins; Plant Proteins; Recombinant Proteins; splicing factor 2
Topics
Beta glucuronidaseGOS2 protein, Oryza sativaSplicing factor 2Vegetable proteinBiosynthesisComparative studyGeneticsMetabolismMolecular cloningMolecular geneticsPromoter regionRiceAmino Acid SequenceBase SequenceCloning, MolecularComparative StudyGenes, PlantGenetic EngineeringGlucuronidaseMolecular Sequence DataNuclear ProteinsOryza sativaPlant ProteinsPlantsPromoter Regions (Genetics)Recombinant ProteinsRestriction MappingSequence Homology, Amino AcidSupport, Non-U.S. Gov'tTissue Distribution
TNO Identifier
231994
ISSN
09607412
Source
Plant Journal, 2(6), pp. 837-844.
Pages
837-844
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