An upstream activating sequence from the Aspergillus nidulans gpdA gene
article
Introduction of a previously identified promoter element of the Aspergillus nidulans gpdA gene (encoding glyceraldehyde-3-phosphate dehydrogenase), the so-called gpd box, into the upstream region of the highly regulated A. nidulans amdS gene (encoding acetamidase), significantly increased (up to 30-fold) the expression of the lacZ reporter gene fused to these expression signals. This increase was dependent on the orientation of the gpd box and on the site of introduction into the amdS upstream region. The presence of additional gpdA sequences which flank the gpd box reduced or even extinguished positive effects of the gpd box. ω-Amino acid and carbon catabolite regulation of the amdS promoter were retained after introduction of the gpd box, indicating that the gpd box does not abolish interactions of the regulatory proteins, AmdR and CreA, with the amdS transcription control sequences. Based on these results, it is suggested that the gpd box comprises at least two separate activities: one being orientation dependent, but relatively independent of position of the gpd box in the upstream region, and the other is only functional near other sites of transcripitonal control. Most likely, both activities are not involved in regulation of the amdS promoter.
Topics
ω-Amino acid inductionamdS::lacZ fusion geneCarbon catabolite repressionNitrogen metabolite repressionRecombinant DNATargeted single-copy integrationCarbonNitrogenAspergillus nidulansCatabolismGene expressionGenetic regulationNonhumanNucleotide sequencePriority journalPromoter regionReporter geneAspergillus nidulansBase SequenceGene Expression Regulation, FungalGenetic VectorsGlyceraldehyde-3-Phosphate DehydrogenasesMolecular Sequence DataPromoter Regions (Genetics)Regulatory Sequences, Nucleic AcidAspergillusEmericella nidulans
TNO Identifier
231951
ISSN
03781119
Source
Gene, 120(1), pp. 67-73.
Pages
67-73
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