The isolated blood-perfused pig ear: An inexpensive and animal-saving model for skin penetration studies
article
To overcome most of the disadvantages of current models to investigate percutaneous penetration of drugs or toxic substances, a model is proposed here based on the isolated pig ear, which is obtained at the slaughterhouse, and perfused with oxygenated blood from the same pig. To determine the viability of the preparations, we measured glucose consumption and lactate production as metabolic parameters, Na+ and K+ ions, as well as lactate dehydrogenase activity in blood as markers for cell damage, whereas vasomotor reactivity was assessed by administering noradrenalin or isoxsuprine. After 60 min of equilibration, only insignificant changes in these parameters were observed during the subsequent 3-hr test period (longer periods were not tested). A slight weight increase was noted during the total period of 4 hr, presumably due to slight edema formation. On the basis of several types of measurements, such as in vivo blood flow and ear temperature and in vitro glucose metabolism, standard procedures were developed. It is concluded that this technique offers an easy to handle, cost-efficient, and animal-saving model for skin penetration studies that lacks most of the disadvantages of existing models. Chemicals/CAS: Blood Glucose; Lactate Dehydrogenase, EC 1.1.1.27; Potassium, 7440-09-7; Sodium, 7440-23-5
Topics
Glucose consumptionIn vitro dermatotoxicityIsolated pig earPorcine skinSkin penetration modelsGlucoseIsoxsuprineLactate dehydrogenaseLactic acidNoradrenalinPotassium ionSodium ionAnimal experimentAnimal tissueBlood vessel reactivityControlled studyEarGlucose transportIsolated organMetabolismModelNonhumanPerfusionPerfusion pressurePriority journalSkin penetrationSwineTechniqueToxicologyAnimalAnimal Testing AlternativesBloodBlood GlucoseCosts and Cost AnalysisEar, ExternalLactate DehydrogenaseModels, BiologicalOsmolar ConcentrationPerfusionPotassiumSkin AbsorptionSodiumSupport, Non-U.S. Gov'tTime FactorsToxicologyVasoconstrictionAnimaliaSuidaeSus scrofa
TNO Identifier
231878
ISSN
10568719
Source
Journal of Pharmacological and Toxicological Methods, 27(2), pp. 71-77.
Pages
71-77
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