Visualization of proteases within a complex sample following their selective retention on immobilized bacitracin, a peptide antibiotic

article


Noort, J.M. van

Berg, P. van den

Mattern, I.E.
A method for the visualization of individual proteases within a complex biological sample is described. In a single chromatographic step, proteases can be separated from other biomolecules by selective binding to immobilized bacitracin, a peptide antibiotic. Following desorption, proteases may be separated by SDS-polyacrylamide gel electrophoresis. The application of this method is presented in the visualization of proteases secreted by the fungus Aspergillus niger.
Topics

















BacitracinProteinaseAspergillus nigerChromatographyNonhumanPolyacrylamide gel electrophoresisPriority journalProtein analysisProtein electrophoresisAspergillus nigerBacitracinElectrophoresis, Polyacrylamide GelEndopeptidasesPeptidesProtein BindingSepharoseAspergillus nigerFungi
TNO Identifier
231618
Repository link
https://resolver.tno.nl/uuid:098e5e30-e832-4b3e-88da-aef9943c9eb9
ISSN
0003-2697
Source
Analytical Biochemistry, 198(2), pp. 385-390.
Pages
385-390
Files
To receive the publication files, please send an e-mail request to TNO Repository.