Determination of several retinoids, carotenoids and E vitamers by high-performance liquid chromatography. Application to plasma and tissues of rats fed a diet rich in either β-carotene or canthaxanthin
article
A method, using two different systems, is described for the high-performance liquid chromatographic analysis of retinol, retinal, retinoic acid, retinyl acetate, retinyl palmitate, α-, β- and γ-carotene, β-apo-6'-β-apo-8'-, β-apo-10'- and β-apo-12'-carotenal, ethyl β-apo-8'-carotenoate, α-tocopherol and α-tocopheryl acetate. The first system consists of a laboratory-packed Hypersil-ODS 3-μm column and a mobile phase of acetonitrile-methylene chloride-methanol-water (70:10:15:5, v/v). The second system consists of a laboratory-packed Nucleosil C18 3-μm column and a mobile phase of acetonitrile-0.1 M ammonium acetate (80:20, v/v). The detection limits in standard were 10 ng/ml for retinoids and carotenoids and 60 ng/ml for the E vitamers. Analysis of the tissues and plasma of rats, after 2 weeks on a diet supplemented with either β-carotene or canthaxanthin (both 2 mg/g), led to the conclusion that the rats were able both to transport and store β-carotene and canthaxanthin and to convert β-carotene to retinol. Incubation of cytosol preparations from the mucosa of the small intestine of rat with 1 μg of β-carotene resulted in the formation of 10-20 ng of retinal within 1 h. Chemicals/CAS: beta Carotene, 7235-40-7; Canthaxanthin, 514-78-3; Carotenoids, 36-88-4; Retinoids; Vitamin E, 1406-18-4
Topics
TNO Identifier
231592
ISSN
00219673
Source
Journal of Chromatography, 553(1-2), pp. 179-186.
Pages
179-186
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