Abscisic acid induces a cytosolic calcium decrease in barley aleurone protoplasts

article


Wang, M.

Duijn, B. van

Schram, A.W.
Cytosolic calcium concentrations (Ca(i)) of barley aleurone protoplasts after stimulation with the plant hormone abscisic acid (ABA) were measured by using the calcium-sensitive fluorescent dye Indo-1. The measuredd basal Ca(i) is about 200 nM. Stimulation with ABA induces a strong dose-dependent decrease in Ca(i) to a minimal value of about 50 nM. This decrease occurs within 5 s. The Ca2+ antagonists La3+ and Cd2+ inhibit the ABA-induced Ca(i) decrease in a dose-dependent manner, while the Ca2+ channel blockers verapamil and nifedipine give no inhibition. The induction of Ca(i) decrease by ABA is consistent with activation of the plasma membrane Ca2+-ATPase by ABA. The possible role of this ABA-induced Ca(i) decrease in ABA signal transduction and in counteracting the effects of gibberellic acid are discussed. Chemicals/CAS: Histocompatibility Antigens Class IIChemicals/CAS: Abscisic Acid, 21293-29-8; Ca(2+)-Transporting ATPase, EC 3.6.1.38; Calcium, 7440-70-2; Fluorescent Dyes; indo-1, 96314-96-4; Indoles; Nifedipine, 21829-25-4; Verapamil, 52-53-9
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Barley aleuroneDormancyGibberellic acidCadmiumLanthanidePlantAbscisic AcidCa(2+)-Transporting ATPaseCalciumCell MembraneCytosolEnzyme ActivationFluorescent DyesHordeumIndolesNifedipineProtoplastsSupport, Non-U.S. Gov'tVerapamilHordeum vulgare subsp. vulgare
TNO Identifier
231480
Repository link
https://resolver.tno.nl/uuid:695f1328-8271-4aca-8874-212e3dfa9a58
ISSN
00145793
Source
FEBS Letters, 278(1), pp. 69-74.
Pages
69-74
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