Induction of type II collagen-specific antibody production in blood lymphocyte cultures of Rhesus monkeys (Macaca mulatta) with collagen-induced arthritis using the immobilized native antigen

article


Hart, B.A. 't

Botman, C.A.D.

Bakker, N.P.M.
Peripheral blood mononuclear cells (PBMC) from Rhesus monkeys previously immunized with bovine type II collagen to induce arthritis were cultured with the same antigen. Because the native protein is poorly soluble in culture medium a heating step is often used. The antigen in this form induced PBMC proliferation, but epitopes for the induction of antibody production and arthritis were lost. To keep the native protein intact, it was coated on affigel beads. With the immobilized antigen specific antibody production could be induced.
Topics

























Antibody productionPeripheral blood mononuclear cellsType II collagencollagen type 2agedanimal cellanimal modelantibody productionarthritisarticlecontrolled studyimmunizationmonkeynonhumanpriority journalAnimalAntibody SpecificityArthritisAutoantibodiesCollagenDisease Models, AnimalHeatImmunizationLymphocytesMacaca mulattaSepharose
TNO Identifier
231453
Repository link
https://resolver.tno.nl/uuid:b3729fd3-a15c-42c7-80a9-5c2286385a98
ISSN
00099104
Source
Clinical and Experimental Immunology, 83(3), pp. 375-378.
Pages
375-378
Files