The sequence γ-(312-324) is a fibrin-specific epitope
article
Fibrin accelerates the activation of plasminogen catalyzed by tissue-type plasminogen activator much stronger than fibrinogen. Detailed studies showed that (part of) this rate-enhancing effect of fibrin is brought about by two sites in the fibrin molecule: one in Aα-(148-160) and one in the γ-chain stretch 311-379 (also known as FCB-5). During the fibrinogen-to-fibrin conversion, Aα-(148-160) appears to become accessible, because a monoclonal antibody against synthetic Aα-(148-160) reacts with fibrin, but not with fibrinogen. Because a similar situation may exist for (at least parts of) FCB-5, we have prepared a monoclonal antibody against a part (ie, γ-[312-324]) of FCB-5, and found that this is fibrin-specific and does not bind fibrinogen. We conclude that γ-(312-314) is hidden in fibrinogen and is exposed by the formation of fibrin. Chemicals/CAS: fibrin, 9001-31-4; fibrinogen, 9001-32-5; Amino Acids; Antibodies, Monoclonal; Epitopes; Fibrin, 9001-31-4; Peptide Fragments
Topics
TNO Identifier
231435
ISSN
00064971
Source
Blood, 77(10), pp. 2169-2173.
Pages
2169-2173
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