mRNA levels and methylation patterns of the tyrosine aminotransferase gene in aging inbred rats
article
We have examined the mRNA levels and methylation patterns of the liver-specific tyrosine aminotransferase (TAT) gene in inbred female rats aged 6, 24 and 36 months. Northern hybridization analysis of total RNA showed a 65% decrease in the steady state transcript level of TAT in the liver of 24- and 36-month old rats as compared to 6-month old animals. The TAT gene as studied by Southern hybridization analysis using the isoschizomers Hpa II and Msp I was found to be hypomethylated in the liver as compared to spleen and brain at six CpG sites within the gene. Methylation at these sites remained unchanged during aging.
Topics
TNO Identifier
231214
ISSN
00145793
Source
FEBS Letters, 269(1), pp. 128-130.
Article nr.
08746
Pages
128-130