Thrombus detection using a Tc-99m labelled antifibrin monoclonal antibody (MoAb) experiments in vitro and in animals

article


Wasser, M.N.J.M.

Pauwels, E.K.J.

Nieuwenhuizen, W.
This paper describes the production and characterization of an antifibrin monoclonal antibody, produced by immunizing mice with fibrinogen degradation fragment by the antibody (designated Y22) is directed against a conformation dependent epitope in the D-domain of fibrin Y22 was labeled with the radionuclide Tc-99m. Experiments in vitro and in rats are presented which show the potential of scintigraphic detection of thrombi with Tc-99m-Y22. Preliminary results show that immunoscintigraphy of thrombi may also have potential for the monitoring of thrombolytic therapy (e.g. with t-PA)
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AnimalAntibodies, MonoclonalCross ReactionsFibrinFibrinolysisHumanImmunoenzyme TechniquesIn VitroMaleMiceMice, Inbred BALB CMicroscopy, FluorescenceRatsRats, Inbred StrainsTechnetiumThrombosisVena Cava, Inferior
TNO Identifier
231056
Repository link
https://resolver.tno.nl/uuid:0f06936c-2e7f-4ac0-853c-fe0c7c4043dc
ISSN
00493848
Source
Thrombosis Research, 57(SUPPL. 10), pp. 91-104.
Pages
91-104
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