Protein synthesis inhibition by cycloheximide does not affect the acute release of tissue-type plasminogen activator
article
The acute release of tissue-type plasminogen activator (t-PA) was studied in perfused rat hindlegs. Pretreatment of rats with the protein synthesis inhibitor cycloheximide (2 mg/kg) at 1, 3 or 5 h prior to perfusion of rat hindlegs did not influence the amount of t-PA released by platelet-activating factor (20 nM) or bradykinin (1 μM). The amount of t-PA activity that could be extracted from hindleg skeletal muscle was not decreased by cycloheximide pretreatment though it was decreased in lung extracts. The in vivo release of t-PA was not affected by cycloheximide pretreatment. The data suggest that the acute release of t-PA from vascular endothelial cells does not require ongoing protein synthesis, but that acutely released t-PA is derived from a stable endothelial storage pool. Chemicals/CAS: cycloheximide, 642-81-9, 66-81-9; plasminogen activator, 9039-53-6; Cycloheximide, 66-81-9; Protein Synthesis Inhibitors; Tissue Plasminogen Activator, EC 3.4.21.68
Topics
TNO Identifier
230886
ISSN
03406245
Source
Thrombosis and Haemostasis, 61(3), pp. 442-447.
Pages
442-447
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