An antifibrin monoclonal antibody useful in immunoscintigraphic detection of thrombi
article
Balb/c mice were immunized with human plasmin-generated fibrinogen degradation product Y. Spleen cells were fused with P3X63-Ag8.653 myeloma cells. A clone (Y22) was found that produces monoclonal antibodies (MoAbs) with a strong reactivity with human fibrin and only a weak reactivity with fibrinogen in an enzyme immunoassay (EIA). Y22 also reacts with fibrin of rabbits, rats, sheep, and dogs. The antibodies are of the IgG1 κ-type and appear to be directed against a conformation-dependent epitope in the D-domain of fibrin. Experiments with 99mTc-labeled Y22 in vitro show that Y22 binds rapidly to forming clots. 99mTc-Y22 also binds to performed plasma clots in a plasma milieu, even in the presence of high concentrations of heparin. Clot localization experiments in rabbits and rats confirm the high fibrin specificity and the potential of 99mTc-Y22 for thrombus imaging in vivo. Chemicals/CAS: fibrin, 9001-31-4; technetium 99m, 14133-76-7; Antibodies, Monoclonal; Binding Sites, Antibody; Fibrin, 9001-31-4; Fibrinogen, 9001-32-5; Technetium, 7440-26-8
Topics
Fibrinogen degradation productMonoclonal antibodyTechnetium 99mAnimal cellHumanHuman cellImmunoscintigraphyMouseNonhumanNormal humanThrombosisAbdomenAnimalAntibodies, MonoclonalBinding Sites, AntibodyFibrinFibrinogenImmunoenzyme TechniquesJugular VeinsMaleMiceMice, Inbred BALB CRabbitsRatsSupport, Non-U.S. Gov'tTechnetiumThrombosisVena Cava, Inferior
TNO Identifier
230780
ISSN
00064971
Source
Blood, 74(2), pp. 708-714.
Pages
708-714
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