Determination of total volatile bases in fish: A collaborative study by the West European Fish Technologists' Association (WEFTA)
article
Two series of collaborative tests were performed. Nine WEFTA laboratories participated in the first exercise, six in the second one. All laboratories applied a common method involving the precipitation of fish proteins by trichloroacetic acid followed by distillation of the extract after addition of sodium hydroxide ("Codex method"). They also carried out the TVB (total volatile bases) analyses by their own methods ("home methods"). Mackerel and cod were used. The results of both collaborative exercises showed important systematic errors between participating laboratories both with the codex and with the home methods. Better comparability was obtained when pure solutions of ammonia, di- and trimethyl-amine (the main components of TVB) were subjected to the same methods. On the other hand, the addition of TMA showed average recoveries of only 75% with significant variability among collaborators. The analytical methods should be further scrutinized and the effects of minor but possibly important variations should be investigated before proposing a reference TVB method. ?? 1987 Springer-Verlag.
TNO Identifier
230389
ISSN
00443026
Source
Zeitschrift fur Lebensmittel-Untersuchung und -Forschung, 184(2), pp. 110-114.
Pages
110-114
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