Production and characterization of a monoclonal antibody reactive with a specific neoantigenic determinant (comprising B?? 54-118) in degradation products of fibrin and of fibrinogen
article
Balb/c mice were immunized with a mixture of fibrin degradation products (XDPs) prepared by complete lysis of a human bloot clot by tissue-type plasminogen activator and purified by immunoaffinity chromatography. Spleen cells of the mice were fused with P3 x 63 Ag 8653 myeloma cells. A clone (FDP 14) was selected that produces monoclonal antibodies (MoAbs) of the IgG1?? type that react with a neoantigenic determinant exposed in these XDPs, but not in intact fibrinogen or in fibrin monomers. Furthermore, the MoAb is reactive with some pure, individual degradation products of fibrinogen (fragments X, Y, E, and the N-terminal disulphide knot) and with the fibrinogen B??-chain but not with A??- and ??-chains or with fragments D, FCB-2 and FCB-3. Comparison of the known primary structures of these fibrinogen fragments indicates that the stretch B?? 54-118 comprises at least an important part of the epitope recognized by FDP-14. Apparently, this stretch contributes importantly to a neoantigenic determinant that is not functional in intact fibrinogen and fibrin monomer and that can be made functional by reduction of fibrinogen, or by digestion with plasmin or CNBr.
Chemicals/CAS: fibrin, 9001-31-4; fibrinogen, 9001-32-5; Antibodies, Monoclonal; Fibrin Fibrinogen Degradation Products; Fibrin, 9001-31-4; Fibrinogen, 9001-32-5
Chemicals/CAS: fibrin, 9001-31-4; fibrinogen, 9001-32-5; Antibodies, Monoclonal; Fibrin Fibrinogen Degradation Products; Fibrin, 9001-31-4; Fibrinogen, 9001-32-5
Topics
Monoclonal antibodyBlood and hemopoietic systemEnzyme linked immunosorbent assayFibrinogen b beta chainFibrinolysisMouseNeoantigenNonhumanAnimalAntibodies, MonoclonalAntibody FormationAntibody SpecificityAntigen-Antibody ReactionsFemaleFibrinFibrin Fibrinogen Degradation ProductsFibrinogenHumanMiceMice, Inbred BALB C
TNO Identifier
230047
ISSN
00064971
Source
Blood, 68(2), pp. 437-441.
Pages
437-441
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