Identification and characterization of the furfural and 5-(hydroxymethyl)furfural degradation pathways of Cupriavidus basilensis HMF14
article
The toxic fermentation inhibitors in lignocellulosic hydrolysates pose significant problems for the production of second-generation biofuels and biochemicals. Among these inhibitors, 5-(hydroxymethyl) furfural (HMF) and furfural are specifically notorious. In this study, we describe the complete molecular identification and characterization of the pathway by which Cupriavidus basilensis HMF14 metabolizes HMF and furfural. The identification of this pathway enabled the construction of an HMF and furfural-metabolizing Pseudomonas putida. The genetic information obtained furthermore enabled us to predict the HMF and furfural degrading capabilities of sequenced bacterial species that had not previously been connected to furanic aldehyde metabolism. These results pave the way for in situ detoxification of lignocellulosic hydrolysates, which is a major step toward improved efficiency of utilization of lignocellulosic feedstock.
Topics
DegradationHydolysateHydroxymethyl furfuralInhibitorsLignocellulosic5 (hydroxymethyl)furfuralaldehyde derivativefuran derivativefurfurallignocelluloseprotein hydrolysateunclassified drugarticlebacterial genebacterial strainbacterium identificationcarbon sourcecatabolismcontrolled studyCupriavidusCupriavidus basilensisdetoxificationgene sequencemicrobial degradationnonhumannucleotide sequencepriority journalPseudomonas putidaCupriavidusDNA Transposable ElementsFuraldehydeGenes, BacterialMetabolic Networks and PathwaysMutagenesisOxidoreductasesPhenotypePseudomonas putida
TNO Identifier
347462
ISSN
00278424
Source
Proceedings of the National Academy of Sciences of the United States of America, 107(11), pp. 4919-4924.
Pages
4919-4924
Files
To receive the publication files, please send an e-mail request to TNO Repository.