PAF-acether-induced release of tissue-type plasminogen activator from vessel walls
article
Platelet-activating factor (PAF-acether; 1-O-octadecyl-2-acetyl-sn-glyceryl-3-phosphorylcholine) induced the release of plasminogen activator in rat, both in vivo and in perfused hind legs. The released plasminogen activator was shown by immunologic and functional criteria to be tissue-type plasminogen activator (t-PA). Release of t-PA by PAF-acether could be inhibited by phospholipase inhibitors and by lipoxygenase inhibitors, but not by cyclooxygenase inhibitors. It is suggested that PAF-acether induces the release of t-PA from vascular endothelial cells by the (calcium-dependent) activation of a phospholipase-lipoxygenase pathway. Chemicals/CAS: 3 amino 1 (3 trifluoromethylphenyl) 2 pyrazoline, 66000-40-6; 4 bromophenacyl bromide, 99-73-0; acetylsalicylic acid, 493-53-8, 50-78-2, 53663-74-4, 53664-49-6, 63781-77-1; diethylcarbamazine, 1642-54-2, 1851-24-7, 90-89-1; docebenone, 80809-81-0; edetic acid, 150-43-6, 60-00-4; indometacin, 53-86-1, 74252-25-8, 7681-54-1; mepacrine, 69-05-6, 83-89-6; nordihydroguaiaretic acid, 500-38-9; plasminogen activator, 9039-53-6; thrombocyte activating factor, 64176-80-3, 65154-06-5; tissue plasminogen activator, 105913-11-9; Calcium, 7440-70-2; Cyclooxygenase Inhibitors; Lipoxygenase Inhibitors; Phospholipases, EC 3.1.-; Plasminogen Activators, EC 3.4.21.-; Platelet Activating Factor
Topics
3 amino 1 (3 trifluoromethylphenyl) 2 pyrazoline4 bromophenacyl bromideAcetylsalicylic acidDiethylcarbamazineDocebenoneEndetic acidIndometacinMepacrineNordihydroguaiaretic acidPlasminogen activatorThrombocyte activating factorTissue plasminogen activatorAnimal experimentBiological modelBlood and hemopoietic systemBlood clottingBlood vessel wallCardiovascular systemDose responseDrug accumulationDrug analysisDrug blood levelDrug comparisonDrug determinationDrug identificationDrug inhibitionDrug isolationDrug mechanismDrug responseHistologyIntravenous drug administrationNonhumanPeripheral vascular systemRegional perfusionSpectrophotometryThrombocyte aggregating factorAnimalBlood VesselsCalciumCyclooxygenase InhibitorsFibrinolysisLipoxygenase InhibitorsMalePhospholipasesPlasminogen ActivatorsPlatelet Activating FactorRatsRats, Inbred StrainsSupport, Non-U.S. Gov't
TNO Identifier
229822
ISSN
00064971
Source
Blood, 66(1), pp. 86-91.
Pages
86-91
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