Fluorometric determination of total vitamin C and total isovitamin C in foodstuffs and beverages by high-performance liquid chromatography with precolumn derivatization
article
A reliable and sensitive high-performance liquid chromatographic (HPLC) method is described for the simultaneous determination of total vitamin C (l-ascorbic acid, AA, plus dehydro-1-ascorbic acid, DHAA) and its C-5 epimer, total isovitamin C (erythorbic acid, EA, plus dehydroerythorbic acid, DHEA), in foodstuffs and beverages. After extraction AA and EA are oxidized enzymatically to DHAA and DHEA with the aid of ascorbate oxidase (EC 1.10.3.3). The latter compounds are condensed with o-phenylenediamine (OPDA) to their highly fluorescent quinoxaline derivatives. These derivatives are separated on a reversed-phase HPLC column and detected fluorometrically. Total vitamin C and isovitamin C can be determined in concentrations as low as 0.2 ??g/g. The amounts of DHAA and DHEA present in foodstuffs and beverages can be determined separately by the same procedure with omission of the enzymatic oxidation. ?? 1984 American Chemical Society.
Topics
TNO Identifier
229645
ISSN
00218561
Source
Journal of Agricultural and Food Chemistry, 32(2), pp. 352-355.
Pages
352-355
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