Urine profile analysis by field desorption mass spectrometry, a technique for detecting metabolites of xenobiotics. Application to 3,5-dinitro-2-hydroxytoluene

article

Greef, J. van der

Leegwater, D.C.
A technique for the detection of biotransformation products of xenobiotics in crude urine extracts by field desorption mass spectrometric profile analysis is described. The method comprises determination of peak profiles of a series of blank and test samples using low resolution field desorption mass spectrometry, comparison of averaged peak profiles and noise reduction by means of Fisher and ratio weighting of peak intensities. Application of the technique to 3,5-dinitro-2-hydroxytoluene has resulted in the detection of two hitherto unknown metabolites in rat urine. By thin-layer co-chromatography, high resolution electron impact mass spectrometry and thin-layer chromatographic-field desorption mass spectrometric analysis they could be identified as 3,5-dinitro-2-hydroxybenzenemethanol and 3,5-diacetamido-2-hydroxytoluene.
Chemicals/CAS: dinitro ortho cresol, 534-52-1; Cresols; Dinitrocresols; hedolit, 534-52-1
Topics



















Dinitro ortho cresolDrug metaboliteRadioisotopeXenobiotic agentDinitro ortho cresol c 14Drug analysisDrug determinationMass spectrometryMethodologyNonhumanThin layer chromatographyUrineAnimalBiotransformationCresolsDinitrocresolsMaleRatsRats, Inbred StrainsSpectrum Analysis, Mass
TNO Identifier
229378
Repository link
https://resolver.tno.nl/uuid:bf487f06-7572-4483-b62d-e51972cf149b
ISSN
0306042X
Source
Biomedical Mass Spectrometry, 10(1), pp. 1-4.
Pages
1-4
Files
To receive the publication files, please send an e-mail request to TNO Repository.