High-performance liquid chromatographic determination of urinary free catecholamines with electrochemical detection after prepurification on immobilized boric acid
article
A reliable high-performance liquid chromatographic method is presented for the determination of the urinary free catecholamines noradrenaline, adrenaline and dopamine. Urine is purified on a column of immobilized boric acid. Catecholamines are separated by ion-pair reversed phase high-performance liquid chromatography and detected electrochemically. The method is suited for routine analysis. It allows the determination of urinary free catecholamines in concentrations as low as 1 ??g/l for noradrenaline and adrenaline and 5 ??g/l for dopamine. A single analysis can be completed within 1 h. Routine analyses can be carried out in a series of 40 samples within 2 days. The within-assay and between-assay coefficients of variation of the analyses in urine were both 2.9% for noradrenaline, both 5.0% for adrenaline, and 1.9 and 2.1% for dopamine. The chromatographic properties of the immobilized boric acid were investigated. In particular, the elution pattern of a series of catecholamine metabolites and analogues was determined. Under the conditions used, only basic compounds containing both a vicinal hydroxyl configuration and a primary or secondary amino group adsorb and elute together with the free catecholamines. Chemicals/CAS: adrenalin, 51-43-4, 55-31-2, 6912-68-1; alpha methyldopamine, 555-64-6; dopamine, 51-61-6, 62-31-7; isoprenaline, 299-95-6, 51-30-9, 6700-39-6, 7683-59-2; noradrenalin, 1407-84-7, 51-41-2; boric acid, 11113-50-1; Boric Acids; Catecholamines
Topics
TNO Identifier
229369
ISSN
00098981
Source
Clinica Chimica Acta, 128(1), pp. 103-113.
Pages
103-113
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