Development and validation of a quantitative method for the determination of 12 endocannabinoids and related compounds in human plasma using liquid chromatography-tandem mass spectrometry
article
A sensitive and specific LC-MS/MS method for the quantification of the endocannabinoids and related structures anandamide, 2-arachidonoyl glycerol, 2-arachidonyl glycerol ether, O-arachidonoyl ethanolamide, dihomo-γ-linolenoyl ethanolamide, docosatetraenoyl ethanolamide, N-arachidonoyl dopamine, N-arachidonyl glycine, N-oleoyl dopamine, oleoyl ethanolamide, palmitoyl ethanolamide, and stearoyl ethanolamide in human plasma was developed and validated. Compounds were extracted using acetonitrile followed by solid-phase extraction. Separation was performed on a Xterra C8 column using gradient elution coupled to a triple-quadrupole MS. LLOQ levels ranged from 0.02 to 1.75 μg/mL, LODs ranged from 0.0002 to 0.1266 ng/mL, and accuracies were >80% (except stearoyl ethanolamide at lowest spike level) at all spike levels.
Topics
EndocannabinoidsHuman plasmaLC-MS/MSSample pre-treatmentGradient elutionsLiquid chromatography-tandem mass spectrometriesRelated compoundsSolid-phase extractionsChromatographic analysisHigh performance liquid chromatographyMass spectrometryPhase separationdiagnostic accuracydrug determinationdrug isolationTandem Mass Spectrometry
TNO Identifier
241537
ISSN
15700232
Source
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 877(14-15), pp. 1583-1590.
Publisher
Elsevier
Place of publication
Amsterdam
Pages
1583-1590
Files
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