Bioconversion of red ginseng saponins in the gastro-intestinal tract in vitro model studied by high-performance liquid chromatography-high resolution Fourier transform ion cyclotron resonance mass spectrometry

article







Kong, H.

Wang, M.

Venema, K.

Maathuis, A.

Heijden, R. van der

Greef, J. van der

Xu, G.

Hankemeier, T.
A high-performance liquid chromatography-high resolution Fourier transform ion cyclotron resonance mass spectrometry (HPLC-FTICR-MS) method was developed to investigate the metabolism of ginsenosides in in vitro models of the gastro-intestinal tract. The metabolites were identified by high-resolution tandem mass spectrometry. Degradation and bioconversion routes of the different ginsenosides at acidic (gastric) conditions and in the presence of intestinal microbiota were elaborated. Besides hydrolysis (deglycosylation) also hydration reactions occurred at acidic conditions. The results illustrate the value of metabolite profiling by HPLC-FTICR-MS for understanding of the mechanisms in bioavailability of herbal drugs and their metabolites. © 2008 Elsevier B.V. All rights reserved.
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Analytical researchBiomedical researchAcidic hydrolysisGinsenosideHPLC-FTICR-MSIntestinal bacteriaMetabolismMetabolite profilingPanax ginsengGastro-intestinal modelTIMAcidic hydrolysisGinsenosideHPLC-FTICR-MSIntestinal bacteriaMetabolite profilingPanax ginsengBacteriologyBiochemistryBiomoleculesBody fluidsChromatographic analysisChromatographyCyclotron resonanceCyclotronsElectron cyclotron resonanceHigh performance liquid chromatographyHigh pressure liquid chromatographyHydrolysisIon chromatographyLiquid chromatographyMass spectrometersMass spectrometryMetabolismMetabolitesPharmacodynamicsPlasmasResonanceSpectrometersSpectrometrySpectrum analysisTechnological forecastingThermal insulating materialsFourier transformsChinese medicineBiological AvailabilityBiotransformationChromatography, High Pressure LiquidFourier AnalysisGinsenosidesHumansHydrogen-Ion ConcentrationIntestine, LargeMass SpectrometryModels, BiologicalPanaxReproducibility of ResultsSolid Phase ExtractionPanax ginseng
TNO Identifier
241441
Repository link
https://resolver.tno.nl/uuid:74f3b8f1-dd32-43c2-86b1-8d11a0ecaaa0
ISSN
00219673
Source
Journal of Chromatography A, 1216(11), pp. 2195-2203.
Pages
2195-2203
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