Determination of the B2 vitamer flavin-adenine dinucleotide in whole blood by high-performance liquid chromatography with fluorometric detection
article
A reliable high-performance liquid chromatographic (HPLC) method is developed for the analysis of FAD in whole blood of humans. The method is able to separate FAD, FMN and Rb from each other and from interfering compounds. A reliable and sensitive detection of FAD has been obtained by selecting the optimal pH of the mobile phase and optimal adjustment of the fluorescence detector.
Chemicals/CAS: flavine adenine nucleotide, 146-14-5; riboflavin, 83-88-5; nicotinamide adenine dinucleotide, 53-84-9; NAD, 53-84-9
Chemicals/CAS: flavine adenine nucleotide, 146-14-5; riboflavin, 83-88-5; nicotinamide adenine dinucleotide, 53-84-9; NAD, 53-84-9
Topics
Flavine adenine nucleotideFlavine nucleotideRiboflavinNicotinamide adenine dinucleotideBloodBlood and hemopoietic systemDrug blood levelDrug determinationFluorometryHigh performance liquid chromatographyHumanHuman cellIn vitro studyMethodologyNormal humanNormal valuePreliminary communicationTheoretical studyArticleChemistrySpectrofluorometryChromatography, High Pressure LiquidHumanMicrochemistryNADSpectrometry, Fluorescence
TNO Identifier
229274
Source
Journal of Chromatography, 228, pp. 311-316.
Pages
311-316
Files
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