An enhancing effect of poly-lysine on the activation plasminogen
article
This study demonstrates that poly-lysine mimics the cofactor (enhancing) function of fibrin that is seen in tissue activator-induced plasminogen activation. Additionally, and in contrast to fibrin, poly-lysine exhibited an enhancing effect on low molecular weight (and not high molecular weight) urokinase-induced plasminogen activation. A mechanism is proposed for this enhancement that involves the rendezvous and local concentration of plasminogen and plasminogen activator molecules on the lysine polymer. All components were able to bind to immobilized poly-lysine making the proposed mechanism feasible. It was possible to elute the activators and mino-plasminogen by high salt (1 M NaCl), in contrast to Glu-plasminogen and plasmin which required 1 M lysine for elution, suggesting specific binding involving lysine binding sites. The enhancing effect was specific for poly-D- and L-lysine and poly-L-ornithine, which has a similar structure. The use of poly-lysine can lead to a better standardization and increased sensitivity of indirect two-step assays for plasminogen activators employing synthetic chromogenic substrates. Chemicals/CAS: chondroitin sulfate, 9007-28-7, 9082-07-9; plasminogen activator, 9039-53-6; plasminogen, 9001-91-6; polyarginine, 24937-47-1, 25212-18-4, 26700-68-5; polylysine, 25104-18-1, 25988-63-0, 33960-24-6, 38000-06-5, 73565-56-7; polyornithine, 24937-49-3, 25104-12-5; protamine sulfate, 9009-65-8; urokinase, 139639-24-0; Fibrinolytic Agents; Peptides; Plasminogen Activators, EC 3.4.21.-; Polylysine, 25104-18-1; polyornithine, 25104-12-5; Sodium Chloride, 7647-14-5; Urinary Plasminogen Activator, EC 3.4.21.73
Topics
chondroitin sulfateplasminogenplasminogen activatorpolyargininepolylysinepolyornithineprotamine sulfatetripeptideurokinaseblood clottingin vitro studyBinding SitesFemaleFibrinolytic AgentsHumanMelanomaMolecular WeightPeptidesPlasminogen ActivatorsPolylysineSodium ChlorideSupport, Non-U.S. Gov'tUrinary Plasminogen ActivatorUterus
TNO Identifier
229189
ISSN
03406245
Source
Thrombosis and Haemostasis, 47(1), pp. 41-45.
Pages
41-45
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