The use of a carbodiimide-containing fixative for the immunohistochemical demonstration of coagulation factor VIII in rat vascular tissue
article
Immunohistochemically, coagulation F-VIII-R:AG in vascular endothelial cells can be demonstrated with antihuman F-VIII-R:AG antisera after fixation with 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide, using both peroxidase and fluorescence techniques. The generally used aldehyde containing fixatives, result in a loss of immunoreactivity. Carbodiimide is preferable as a fixative for the cellular localization of F-VIII-R:AG. Chemicals/CAS: blood clotting factor 8, 9001-27-8; cyanamide, 151-51-9, 420-04-2; Ethyldimethylaminopropyl Carbodiimide, 1892-57-5; Factor VIII, 9001-27-8; Fixatives
Topics
TNO Identifier
229157
Source
Histochemistry, 71(2), pp. 187-194.
Pages
187-194
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