Factors influencing the structure of terminal plasmin degradation products of human fibrinogen and fibrin
article
Experiments have been carried out with fibrinogen and with purified degradation products of fibrinogen and fibrin which demonstrate that the structure of D fragments obtained after prolonged plasmin digestion is influenced by several factors in the media. The previously described protective effect of calcium ions on the ??-chain carboxy-terminals of fibrinogen against attack has been confirmed by working at high plasmin concentrations and/or in the presence of 2 M urea. Several compounds such as EDTA, EGTA, citrate and iminodiacetic acid appear to have a separate effect. In the absence of calcium ions these compounds appear to make the ??-chain carboxy-terminal ends of the D and D-dimer fragments more susceptible to plasmin digestion. Finally, as demonstrated by experiments with purified D-E complexes from fibrinogen and with whole fibrinogen digests, the E moiety of the D-E complexes appears to be capable of protecting the D moiety against low plasmin concentrations also in the absence of calcium ions. Chemicals/CAS: fibrinogen, 9001-32-5; plasmin, 9001-90-5, 9004-09-5; Calcium, 7440-70-2; Edetic Acid, 60-00-4; Egtazic Acid, 67-42-5; Fibrin Fibrinogen Degradation Products; Macromolecular Systems; Plasmin, EC 3.4.21.7
Topics
TNO Identifier
228989
ISSN
00063002
Source
Biochimica et Biophysica Acta, 667(2), pp. 321-327.
Pages
321-327
Files
To receive the publication files, please send an e-mail request to TNO Repository.