Pitfalls in determination of acetylcholine from brain by pyrolysis gas chromatography/mass spectrometry

article

Polak, R.L.

Molenaar, P.C.
Demethylation by sodium benzenethiolate has been used to analyse mixtures of isotopic variants of acetylcholine (ACh) by gas chromatography/mass spectrometry (GC/MS). The present experiments show that demethylation by pyrolysis is also suitable for the simultaneous determination of deuterium labelled variants of ACh by means of GC/MS. However, care must be taken that the samples do not contain appreciable amounts of choline, since exchange of deuterium labelled groups between ACh and choline during pyrolysis may yield erroneous results.
Chemicals/CAS: acetylcholine, 51-84-3, 60-31-1, 66-23-9; choline, 123-41-1, 13232-47-8, 1927-06-6, 4858-96-2, 62-49-7, 67-48-1; Acetylcholine, 51-84-3; Carbon Radioisotopes; Deuterium, 7782-39-0; Tritium, 10028-17-8
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AcetylcholineCholineBrainDemethylationGas chromatographyMass spectrometryMethodologyPyrolysisAcetylcholineAnimalBrain ChemistryCarbon RadioisotopesChromatography, GasComparative StudyDeuteriumEvaluation StudiesHeatMethodsSpectrum Analysis, MassTritium
TNO Identifier
227653
Repository link
https://resolver.tno.nl/uuid:d352baa1-8e91-429d-a52f-f224654fd707
ISSN
00223042
Source
Journal of Neurochemistry, 23(6), pp. 1295-1297.
Pages
1295-1297
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