Messungen von Elektrolyten in Nierenschnitten mit der Mikrosonde [Electron microprobe analysis of electrolytes in kidney slices]
article
The intracellular distribution of Na, K and Cl has been investigated by electron probe analysis in different regions and different tubular segments of rat kidneys. Kidneys of antidiuretic rats were frozen in liquid propane at -170° C. Sections of 6-7 μm thickness were cut in a cryostate at -20° C, fixed on a thin nylon foil specimen holder and freeze dried at -40° C. Data were evaluated according to the "relativmethod" of Hall and coworkers. Furthermore data were obtained from thin NaCl, sanidin and several Agar-Agar solution standards for a quantitative evaluation according to the "absolute method" of Marshall and Hall. Using the relativ method the following results were obtained: 1. The intracellular concentrations of sodium and chlorine increase from the superficial layers of the renal cortex towards deeper layers of the cortex. 2. A more pronounced increase of the intracellular concentrations of sodium and chlorine in the outer zone of the renal medulla was found as compared to the cortex. 3. Distinct from sodium and chlorine no cortico-medullary gradient for potassium was observed. 4. Within the outer zone of the renal medulla a statistically significant gradient for Na and Cl has been found between the descending and the ascending limb of Henle's loop. These results are in agreement with the suggested hypothesis of renal countercurrent mechanisms. © 1971 Springer-Verlag. Chemicals/CAS: Electrolytes
Topics
TNO Identifier
227279
ISSN
00263672
Source
Mikrochimica Acta, 59(5), pp. 697-716.
Publisher
Springer-Verlag
Pages
697-716
Files
To receive the publication files, please send an e-mail request to TNO Repository.