Induction of cytochrome formation and stimulation of oxidative dissimilation by hemin in Streptococcus lactis and Leuconostoc mesenteroides
article
Aerobic glucose dissimilation of washed cells of Streptococcus lactis grown in peptone-glucose-yeast extract medium is characterized by the formation of large amounts of lactic acid, a small amount of acetic acid, and traces of acetoin: a corresponding amount of oxygen is taken up. Aerobic metabolism by washed cells of S. lactis and Leuconostoc mesenteroides is far more oxidative when the cells have been grown on peptone-glucose-yeast extract agar supplied with 10 ppm of hemin than when they have been grown in the absence of hemin. In the former case respiration is strongly inhibited by KCN and only slightly by bis(tributylgermanium) oxide, (Bu3Ge)2O. Respiration of cells grown without hemin, on the other hand, is strongly inhibited by (Bu3Ge)2O but only moderately by KCN. In cells grown in the presence of hemin, spectra of an a2- and b-type cytochrome were recognized but not in cells grown without hemin. The NADH-oxidase activity of such cells is not affected by KCN. Our results strongly suggest that by growth in the presence of hemin a cytochrome-mediated respiration system is induced which replaces, in part, the NADH-oxidase-mediated respiration. Whereas the latter is sensitive to (Bu3Ge)2O, the former apparently is little or not. However it is quite sensitive to KCN. When hemin is added to washed cells of S. lactis grown without hemin the rate of oxygen uptake increases immediately though no cytochromes are present and respiration remains sensitive to (Bu3Ge)2O. Possibly hemin stimulates the NADH-oxidase activity of these cells. © 1970 Swets & Zeitlinger. Chemicals/CAS: carbon dioxide, 124-38-9, 58561-67-4; cyanide, 57-12-5; germanium, 7440-56-4; glucose, 50-99-7, 84778-64-3; heme, 14875-96-8; oxide, 16833-27-5; oxidoreductase, 9035-73-8, 9035-82-9, 9037-80-3, 9055-15-6; oxygen, 7782-44-7; potassium, 7440-09-7; Acetic Acids; Butanones; Carbon Dioxide, 124-38-9; Cyanides; Cytochromes; Germanium, 7440-56-4; Glucose, 50-99-7; Glycols; Heme, 14875-96-8; Lactates; Oxides; Oxidoreductases, EC 1.; Oxygen, 7782-44-7; Potassium, 7440-09-7
Topics
Acetic acid derivativeAlkanoneCarbon dioxideCyanideCytochromeGermaniumGlucoseGlycolHemeLactic acid derivativeOxideOxidoreductaseOxygenPotassiumBiosynthesisChemical phenomenaDrug effectEnzymologyLeuconostocMetabolismOxidation reduction reactionOxygen consumptionSaccharomycesStreptococcusAcetic AcidsButanonesCarbon DioxideCyanidesCytochromesDepression, ChemicalGermaniumGlucoseGlycolsHemeLactatesLeuconostocOxidation-ReductionOxidesOxidoreductasesOxygenOxygen ConsumptionPotassiumSaccharomycesStreptococcus
TNO Identifier
227208
ISSN
00036072
Source
Antonie van Leeuwenhoek, 36(1), pp. 335-348.
Publisher
Kluwer Academic Publishers
Pages
335-348
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