The transition between two forms of bacteriophage φX17A differing in heat sensitivity and adsorption characteristics
article
If cells of the bacterium Escherichia coliC infected with bacteriophage φX174 are lysed artificially in the cold, the phage obtained is relatively heat resistant at temperatures around 65° and unable to adsorb to host cells at 4°. At 37° the phage particles become more thermolabile with respect to inactivation at 65° and, concomitantly, obtain the ability to adsorb to E. coli C in the cold. A minor fraction remaining thermoresistant at 37° does not adsorb to host cells even at this temperature. Optimal conditions for the transition of the resistant into the sensitive form in phosphate buffer are 37°, pH ≤ 7 and a concentration of phosphate ≤= 25 mM. At lower pH and high titers of phage the thermosensitive form changes partly back into the thermoresistant one. © 1967.
Topics
TNO Identifier
226974
ISSN
00426822
Source
Virology, 32(1), pp. 93-102.
Pages
93-102
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