RPLC-lon-trap-FTMS method for lipid profiling of plasma: Method validation And application to p53 mutant mouse model
article
A reversed-phase liquid chromatography-linear ion trap-Fourier transform ion cyclotron resonance-mass spectrometry method was developed for the profiling of lipids in human and mouse plasma. With the use of a fused-core C<sub>8</sub> column and a binary gradient, more than 160 lipids belonging to eight different classes were detected in a single LC-MS run. The method was fully validated and the analytical characteristics such as linearity (R<sup>2</sup>, 0.994-1.000), limit of detection (0.08-1.28 μ/mL plasma), repeatability (RSD, 2.7-7.9%) and intermediate precision (RSD, 2.7-15.6%) were satisfactory-The method was successfully applied to p53 mutant mice plasma for studying some phenotypic effects of p53 expression. © 2008 American Chemical Society.
Topics
Analytical researchFTMSLipid profilingLipidomicsMetabolite profilingMetabolomicsP53 mouse modelProtein p53LipidArticleControlled studyHigh performance liquid chromatographyHumanIon cyclotron resonance mass spectrometryIon trap mass spectrometryLipid analysisLipid blood levelMouseMutational analysisNonhumanPhenotypePriority journalProtein expressionReversed phase liquid chromatographyAnimalBloodCyclotronInfrared spectroscopyLiquid chromatographyMethodologyMouse mutantReproducibilityTandem mass spectrometryMusAnimalsChromatography, LiquidCyclotronsLipidsMiceMice, Mutant StrainsReproducibility of ResultsSpectroscopy, Fourier Transform InfraredTandem Mass SpectrometryTumor Suppressor Protein p53
TNO Identifier
280031
ISSN
15353893
Source
Journal of Proteome Research, 7(11), pp. 4982-4991.
Pages
4982-4991
Files
To receive the publication files, please send an e-mail request to TNO Repository.