Synthesis of ribonucleic acid in regenerating rat liver after partial hepatectomy
article
A technique for the fractionation of RNA by column chromatography on calcium phosphate is described. With this technique the incorporation of labeled inorganic phosphate into RNA of regenerating rat liver was studied. In the cytoplasm a RNA fraction was found, with molecular weight intermediate between that of soluble and microsomal RNA, that had a very high incorporation rate. This fraction occurred only in small amounts in the cytoplasm. In the nucleus relatively more of this type of RNA was present but there the specific activity did not differ from that of nuclear ribosomal RNA. Determination of the relative amount of label in the four RNA nucleotides showed that the fraction with intermediate molecular weight is not a breakdown product of ribosomal RNA. [3H]Cytidine incorporation in the nucleolar regions of the hepatocyte nucleus was demonstrated with autoradiography. Lengthening the period between injection of the isotope and death of the animal led to a decrease of nucleolar labeling coupled with an increase in cytoplasmic labeling. These results support earlier observations, indicating the important role of the nucleus in the synthesis of cytoplasmic RNA. © 1965.
TNO Identifier
226874
ISSN
00052787
Source
BBA Section Nucleic Acids And Protein Synthesis, 95(2), pp. 262-279.
Pages
262-279
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