The reliability of the test of McKenzie, Taylor, and Gilbert for the detection of faecal Escherichia coli strains of animal origin in foods
article
Figures for the response of fresh faecal isolates of E. coli of animal origin to the test of McKenzie et al. - a modification of Eijkman's test - being as yet relatively scarce, 140 strains isolated from man, hog, cow, calf, horse, chicken, dog, rat, cat and mouse were tested. All strains formed indole at 44 C within 48 hr; only one strain produced very little. Seven strains did not form gas from lactose within 48 hr at 44 C but four of these did at 30 C; one of the latter also produced gas at 44 C when the inhibitors used in the standard medium were omitted. The percentage false negatives to be expected in this test was found to be 5 on the average, while it appeared less than 10 for the least favourable group of organisms, viz, those of porcine origin. This degree of accuracy of the test has also been found by earlier authors, be it in some cases with a lower number of isolates. The test seems, therefore, quite acceptable for its purpose, the detection of E. coli type I in foods. © 1963 Swets & Zeitlinger.
TNO Identifier
226821
ISSN
00036072
Source
Antonie van Leeuwenhoek, 29(1), pp. 163-168.
Pages
163-168
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