The reaction of chymotrypsin and diisopropylphosphorofluoridate II. The structure of two DP-substituted peptides from chymotrypsin-DP
article
1. 1. The amino acid sequence of two diisopropylphosphoryl-substituted peptides obtained from α-chymotrypsin-DP by enzyme hydrolysis was established as: glycyl-aspartyl-seryl-glycyl-glycyl-prolyl-leucine and glycyl-aspartyl-seryl-glycyl-glycyl-proline, respectively. 2. 2. The experimental results strongly suggest that the diisopropylphosphoryl group is attached to the hydroxyl group of the seryl residue. 3. 3. The significance of the investigated peptides in respect to the DFP-binding and ester-splitting ability of α-chymotrypsin is discussed. © 1958.
TNO Identifier
226689
ISSN
00063002
Source
BBA - Biochimica et Biophysica Acta, 27(C), pp. 556-563.
Pages
556-563
Files
To receive the publication files, please send an e-mail request to TNO Repository.