Investigations on organic fungicides - III. The influence of essential trace metals upon the fungitoxicity of tetramethylthiuram disulphide and 8-hydroxyquinoline
article
The influence of the trace metals Fe, Zn, Cu, Mn and Mo - known to be essential for the growth of the mould Aspergillus niger van Tieghem - upon the fungitoxicity of tetramethylthiuram disulphide (t.m.t.d.) and of 8-hydroxyquinoline was investigated using the same mould as the test organism. It was found that none of the metals upon addition to the medium reversed the toxicity of t.m.t.d. to any perceptible degree neither when the mould was cultivated in a state of being very sensitive to t.m.t.d. (on a synthetic medium) nor when it was grown in a state of being about a 100 times more resistant to the agent (on a malt agar medium). Addition of Zn, Cu, Mn and Mo did not counteract the antifungal activity of 8-hydroxyquinoline. Fe effected a reverse only when the mould was cultivated in a resistant state, whereas this metal had no influence upon the toxicity of 8-hydroxyquinoline when the mould was cultivated in a sensitive state. The iron salt of 8-hydroxyquinoline showed about the same antifungal activity as free 8-hydroxyquinoline only when the test mould was cultivated under conditions favouring a high sensitivity. Most phenomena encountered in the reversal of toxic action of antimicrobial agents by addition of heavy metal ions are satisfactorily explained by the "availability" factor. It was concluded that the antifungal activity of tetramethylthiuram disulphide and 8-hydroxyquinoline cannot be attributed to their capability of precipitating essential heavy metals. © 1951 Swets en Zeitlinger.
TNO Identifier
226614
Source
Antonie van Leeuwenhoek, 17(1), pp. 58-68.
Pages
58-68
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