Net charge affects morphology and visual properties of ovalbumin aggregates
article
The effect of ovalbumin net charge on aggregate morphology and visual properties was investigated using chromatography, electrophoresis, electron microscopy, and turbidity measurements. A range of differently charged ovalbumin variants (net charge ranging from -1 to -26 at pH 7) was produced using chemical engineering. With increasing net charge, the degree of branching and flexibility of the aggregates decreased. The turbidity of the solutions reflected the aggregate morphology that was observed with transmission electron microscopy. Increasing the stiffness of the aggregates transformed the solutions from turbid to transparent. Artificially shielding the introduced net charge by introducing salt in the solution resulted in an aggregate morphology that was similar to that for low-net-charge variants. The morphology of heat-induced aggregates and the visual appearance of the solutions were significantly affected by net charge. We also found that the morphology of ovalbumin aggregates can be rapidly probed by high-throughput turbidity experiments. © 2008 American Chemical Society.
Topics
Food technologyAgglomerationChemical engineeringChromatographic analysisElectrophoresisMorphologyOptical propertiesTurbidityAggregate morphologiesDegree of branchingNet chargesOvalbuminTransmission electronsTurbidity measurementsVisual appearancesVisual propertiesAggregatesovalbuminarticlechemical modificationcontrolled studydisulfide bondgel permeation chromatographyheatingincubation timemorphologyparticle sizepHphysical chemistrypolyacrylamide gel electrophoresispriority journalprotein aggregationprotein structurerigiditytransmission electron microscopyturbidityAnimalsElectrophoresis, Polyacrylamide GelMicroscopy, ElectronNephelometry and TurbidimetryOvalbuminPliabilityPrecipitationProtein ConformationSolubilityStatic Electricity
TNO Identifier
241083
ISSN
15257797
Source
Biomacromolecules, 9(11), pp. 3165-3172.
Pages
3165-3172
Files
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