β1 integrins regulate chondrogenesis and rock signaling in adipose stem cells

article




Lu, Z.F.

Doulabi, B.Z.

Huang, C.L.

Bank, R.A.

Helder, M.N.
β1 integrins play a controversial role during chondrogenesis. Since the maturation of chondrocytes relies on a signaling switch from cell-cell to cell-matrix interactions, we hypothesized that β1 integrins play a different role at the earlier (mainly cell-cell interaction) from the later stage (mainly cell-matrix interaction) of chondrogenesis. Our data showed: in plain medium, sox9, collagen X, and collagen II gene expressions of ASCs were induced by β1-integrin blockage at day 14. In chondrogenic medium, however, sox 9, sox6, and collagen II gene expression were induced at day 4 but inhibited at day 14. In addition, both β1-integrin blockage and TGF-β1 down-regulated Rock-1 and -2 gene expression and produced the round cells. We concluded that β1 integrins play a more important role at the later stages than earlier stages of chondrogenesis, and that the onset of chondrogenesis promoted by β1-integrin blockage might be through inhibiting Rock signaling. © 2008 Elsevier Inc. All rights reserved.
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Biomedical Researchβ1 integrinsAdipose tissueCell shapeChondrogenesisMesenchymal stem cellsRock signalingbeta1 integrincollagen type 10collagen type 2transcription factor Sox9transforming growth factor beta1DNA binding proteinhigh mobility group proteinimmunoglobulin GRho kinasesox-9 transcription factorSOX6 protein, humantranscription factoradipocytearticlecell interactionchondrogenesiscollagen 2 genecollagen x genecontrolled studydown regulationextracellular matrixgenegene expressionhumanhuman cellhuman tissuepriority journalprotein functionrock 1 generock 2 genesignal transductionSox9 geneadipose tissuecell shapeculture mediumcytologydrug antagonismdrug effectgene expression regulationgeneticsphysiologystem cellAdipose TissueAntigens, CD29Cell ShapeChondrogenesisCollagen Type IICollagen Type XCulture MediaDNA-Binding ProteinsGene ExpressionGene Expression Regulation, DevelopmentalHigh Mobility Group ProteinsHumansImmunoglobulin Grho-Associated KinasesStem CellsTranscription FactorsTransforming Growth Factor beta1
TNO Identifier
240949
Repository link
https://resolver.tno.nl/uuid:8cbe1cc4-b2b9-4511-8080-5b8b8204684c
ISSN
0006291X
Source
Biochemical and Biophysical Research Communications, 372(4), pp. 547-552.
Pages
547-552
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