Transcriptome analysis of a phenol-producing Pseudomonas putida S12 construct: Genetic and physiological basis for improved production
article
The unknown genetic basis for improved phenol production by a recombinant Pseudomonas putida S12 derivative bearing the tpl (tyrosine-phenol lyase) gene was investigated via comparative transcriptomics, nucleotide sequence analysis, and targeted gene disruption. We show upregulation of tyrosine biosynthetic genes and possibly decreased biosynthesis of tryptophan caused by a mutation in the trpE gene as the genetic basis for the enhanced phenol production. In addition, several genes in degradation routes connected to the tyrosine biosynthetic pathway were upregulated. This either may be a side effect that negatively affects phenol production or may point to intracellular accumulation of tyrosine or its intermediates. A number of genes identified by the transcriptome analysis were selected for targeted disruption in P. putida S12TPL3. Physiological and biochemical examination of P. putida S12TPL3 and these mutants led to the conclusion that the metabolic flux toward tyrosine in P. putida S12TPL3 was improved to such an extent that the heterologous tyrosine-phenol lyase enzyme had become the rate-limiting step in phenol biosynthesis. Copyright © 2008, American Society for Microbiology. All Rights Reserved.
Topics
Biomedical Researchlyasephenoltryptophantyrosineamino acid synthesisarticlebacterial genebacterial metabolismbacterial mutationbacterium mutantbioaccumulationbiosynthesisgene disruptiongene expression profilinggene expression regulationgene identificationgene targetingnonhumannucleotide sequencepriority journalPseudomonas putidasequence analysistranscriptomicsGene DeletionGene Expression ProfilingMetabolic Networks and PathwaysMutagenesis, InsertionalPhenolPhenylpyruvic AcidsPseudomonas putidaTryptophanTyrosineTyrosine Phenol-LyaseUp-RegulationPseudomonas putida
TNO Identifier
240737
ISSN
00219193
Source
Journal of Bacteriology, 190(8), pp. 2822-2830.
Pages
2822-2830
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